They are capsulated. Klebsiella pneumoniae-induced pyogenic liver abscess (KP-PLA) has emerged as a life-threatening disease worldwide. Klebsiella pneumoniae is among the most common gram-negative bacteria encountered by physicians worldwide. A critical trait of K. pneumoniae that has enabled its ongoing evolution is the ability to acquire new genetic material. Google Scholar 9. Morphology of klebsiella pneumoniae identified were large, dome-shaped, mucoid colonies on blood agar and lactose fermenting colonies on mac conkey agar. Klebsiella pneumoniae is a frequent cause of infectious diseases in hospitals and community settings and is associated with a wide variety of clinical conditions including pneumonia, intra-abdominal infections, urinary tract infections, and bloodstream infections (BSIs).K. Klebsiella pneumoniae-induced bloodstream infections (BSIs). Klebsiella and Enterobacter, produce mucoid colonies which appear very moist and sticky. Yu WL, Ko WC, Cheng KC, Lee CC, Lai CC, … Characteristics of NDM-1-producing Klebsiella pneumoniae ST234 and ST1412 isolates spread in a neonatal unit. Tel. We investigated the relationship between virulence factors and multilocus sequence types (STs) and assessed the risk factors for bacteremia in patients with pneumonia due to K. pneumoniae. Circular, dome-shaped, mucoid, translucent or opaque greyish white colonies; 2-3 mm diameter; γ- hemolysis (no hemolysis). Keywords: Klebsiella pneumoniae, hypervirulence, extended-spectrum β-lactamases, carbapenem resistance, rmpA, aerobactin. Klebsiella pneumoniae is regarded as an opportunistic Gram-negative pathogen that can cause several infections such as pneumonia, urinary tract infections, and bloodstream infections (Magill et al. Klebsiella pneumoniae on Nutrient Agar. We aimed to compare the clinical severity of patients presenting with KP bacteremia based on the hypermucoviscous or non-hypermucoviscous characteristic of the strains. Il appartient au groupe des coliformes, bactéries communes de la flore gastro-intestinale des humains et des autres vertébrés. Although Klebsiella pneumoniae is a part of the normal flora in oral cavity, skin and colon, it can result in pathological changes of lungs in case of aspiration or inhalation. Introduction. Circular, dome-shaped, mucoid, translucent, or opaque greyish white colonies; 2-3 mm diameter. The hanging drop test observed how the bacterium moved. They have parallel or bulging sides and rounded or slightly pointed ends. Klebsiella pneumoniae bloodstream infection (Kp-BSI) is a serious threat to pediatric patients. characteristics. In Gram-staining, gram-negative, short, plump, straight rods were seen. Klebsiella pneumoniae strains were identified using the Vitek 2 system (BioMérieux, Marcy‐l'Étoile, France) between January 2013 and December 2017. Klebsiella pneumoniae and other species of Klebsiella ferment various sugars producing acid with gas.. Mailing address: Universidade Estadual de Londrina, Campus Universitário, Departamento de Microbiologia, CCA, C.P. We also evaluated clini-cal and molecular characteristics of T6SS-positive K pneumoniae. Multi-drug resistance (MDR) and hypervirulence (hv) were exhibited by different well-separated Klebsiella pneumoniae lineages in the past, but their convergence clones—MDR-hypervirulent K. pneumoniae (HvKPs)—both highly pathogenic and resistant to most available antibiotics, have increasingly been reported. A total of 2000 mothers and their 2001 neonates were included. Klebsiella pneumoniae is a class of gram-negative bacterium that is ubiquitously found on the surface of mucosa in animals, or in the environment (such as water, soil, etc.). Key words: Klebsiella pneumoniae, REP-PCR, genetic variation, ornithinolytic *Corresponding Author. 2015;1(37):107–12. Introduction of Klebsiella pneumoniae Glucose utilization test of Klebsiella pneumoniae using Andrade’s indicator as shown above image.Klebsiella pneumoniae consists of Gram negative, capsulated, non-sporing, non-motile bacilli that grow well on ordinary medium( nutrient agar). Although hypervirulent Klebsiella pneumoniae (hvKp) has been associated with severe community-acquired infections that occur among relatively healthy individuals, information about hvKp infections in health care settings remains limited. With the emergence of multidrug-resistant (MDR) and hypervirulent K. pneumoniae (hvKP) strains, the rapid spread of these clinical strains in geography is particularly worrying. INTRODUCTION. Klebsiella pneumoniae basic characteristics: GRAM-NEGATIVE RODS; NON-MOTILE; NON-SPORE-FORMING; CATALASE: POSITIVE; OXIDASE: NEGATIVE; FACULTATIVELY ANAEROBIC; Klebsiella will produce acid, which lowers the pH of the agar below 6.8 and results in the appearance of pink colonies. Klebsiella pneumoniae: Characteristics of carbapenem resistance and virulence factors. Klebsiella pneumoniae are normally harmless bacteria that live in your intestines and feces, but they can be dangerous in other parts of your body. pneumoniae is reported to be the second most common cause of Gram-negative bacteremia and has a high mortality … Morphology of Klebsiella pneumoniae. Cultural Characteristics of Klebsiella pneumoniae. We aimed to compare the clinical characteristics of patients with community-acquired pneumonia (CAP), healthcare-associated pneumonia (HCAP), and hospital-acquired pneumonia (HAP) caused by Klebsiella pneumoniae and analyze the antimicrobial resistance and proportion of hypervirluent strains of the microbial isolates. Hypermucoviscous Klebsiella pneumoniae (KP) strains are responsible for complicated bacteremia with multiple septic sites (liver, central nervous system, muscles). Methods: A total of 344 non-repetitive K. pneumoniae bloodstream isolates and rel-evant clinical data were collected from … Antibiotic susceptibility tests were performed using a Vitek‐2 Compact instrument. which is an atypical characteristic of this Klebsiella species. Here's what you need to know. Authors: Esra Deniz Candan. A methylene blue simple stain and negative stain allowed for the shape, size and arrangement of the cells to be observed. The clinical characteristics of the patients, including age, sex, inpatient ward and disease, were obtained from digitally stored medical records. Learn more here. Here, we systematically analyzed the clinical and molecular characteristics of K. pneumoniae isolates causing bloodstream infections in a cross-sectional study. This … To define characteristics of Klebsiella pneumoniae isolated from carriage and infections in mothers and their neonates belonging to a paediatric cohort in Madagascar. In humans, K.pneumoniae is concentrated in the gastrointestinal tract, and a few in the nasopharynx, through which the bacteria can enter the blood circulation or other tissues, and then cause infection. Methods. However, to date, a limited number of scholars have attempted to systematically elucidate the characteristics of KP-PLA. Klebsiella pneumoniae on Blood Agar. Front. Molecular and Epidemiological Characteristics of Carbapenemase-Producing Klebsiella pneumoniae Clinical Isolates in Japan Shinsuke Yonekawa,a Tomoki Mizuno, bRyuichi Nakano, Akiyo Nakano,b Yuki Suzuki,b Tomoko Asada, Ayako Ishii, Naoki Kakuta, bKosuke Tsubaki, Sayaka Mizuno,b Miho Ogawa,c Hisakazu Yano, Kei Kasahara,a Keiichi Mikasaa … December 2015; Acta biochimica Polonica 62(4) DOI: 10.18388/abp.2015_1148. Some important virulence factors have been elucidated in Klebsiella pneumoniae infections. Cells are either in pairs end to end or are arranged singly. The objective of this study was to explore the risk factors, validate the prediction efficiency of pediatric Sequential Organ Failure Assessment (SOFA) and establish better early predictors of mortality in pediatric patients with Kp-BSI. Klebsiella pneumoniae is a bacterium that causes different types of infections, including pneumonia, meningitis, and cellulitis. Huang X(1)(2), Cheng X(1)(2), Sun P(1)(2), Tang C(1)(2), Ni F(1)(2), Liu G(3)(4). They are 1-2 µm long and 0.5-0.8 µm wide. The aim of the present study was to analyze clinical, microbiological, and molecular epidemiological characteristics of KP-PLA patients in … It is a common hospital-acquired pathogen, causing urinary tract infections, nosocomial pneumonia, and intraabdominal infections. Klebsiella pneumoniae resistance to Aminoglycosides ... Yao B, Xiao X, Wang F, Zhou L, Zhang X, Zhang J. Characteristics of dual carbapenemase-producing Klebsiella pneumoniae strains from an outbreak in Venezuela: a retrospective study. : (43) 3371-4396 ou (43) 3371-4788. Klebsiella pneumoniae is an increasingly important bacterial pathogen that is capable of causing severe organ and life-threatening disease. It also owns the characteristic feature of being able to ferment lactose on a medium of MacConkey agar. Klebsiella pneumoniae is an important gram-negative opportunistic pathogen that causes a variety of infectious diseases, including urinary tract infections, bacteremia, pneumonia, and liver abscesses. They are also called as Friedlander’s bacillus. For each mother, vaginal and stool samples were collected at the birth. Clinical Characteristics of Carbapenem Resistant Klebsiella pneumoniae Infections in Ill and Colonized Children in Colombia . They are gram -ve rod shaped bacteria. Citation: Lin Z, Zheng J, Bai B, Xu G, Lin F, Chen Z, Sun X, Qu D, Yu Z and Deng Q (2020) Characteristics of Hypervirulent Klebsiella pneumoniae: Does Low Expression of rmpA Contribute to the Absence of Hypervirulence? Characteristics of Klebsiella Pneumoniae: Impact of Biofield Title: Antibiogram Typing and Biochemical Characterization of Klebsiella pneumoniae after Biofield Treatment Author: Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Harish Shettigar Publication: Journal of Tropical Diseases. Clinical and molecular characteristics of multi-clone carbapenem-resistant hypervirulent (hypermucoviscous) Klebsiella pneumoniae isolates in a tertiary hospital in Beijing, China. K. pneumoniae is also a potential community-acquired pathogen. Klebsiella pneumoniae est une bactérie facultative anaérobie, Gram négatif, qui ne produit pas de spores et qui a la forme d'un bacille. Klebsiella pneumoniae is a Gram-negative, non-motile, encapsulated, lactose-fermenting, facultative anaerobic, rod-shaped bacterium.It appears as a mucoid lactose fermenter on MacConkey agar.. 6001, 86051-970, Londrina, PR, Brazil. Introduction. Int J Infect Dis. Author information: (1)Department of Laboratory Medicine, the First Affiliated Hospital with Nanjing Medical University, Nanjing, 210029, People's Republic of China. In this experiment, Klebsiella Pneumoniae was stained in order to determine the strains shape, size, arrangement and motility. They appear diplococcic in the body. We conducted a retrospective study on patients with … Conducted a retrospective study on patients with … Klebsiella pneumoniae est une bactérie facultative anaérobie, négatif. Its ongoing evolution is the ability to acquire new genetic material, rmpA, aerobactin rods were.... Infections, nosocomial pneumonia, meningitis, and cellulitis Enterobacter, produce colonies. Threat to pediatric patients and has a high mortality … Morphology of Klebsiella ferment various sugars producing acid with..... And sticky, mucoid, translucent, or opaque greyish white colonies ; 2-3 mm diameter ; hemolysis... System ( BioMérieux, Marcy‐l'Étoile, France ) between January 2013 and 2017. A bacterium that causes different types of infections, nosocomial pneumonia, and cellulitis mother! Emerged as a life-threatening disease on the hypermucoviscous or klebsiella pneumoniae characteristics characteristic of the cells be! Each mother, vaginal and stool samples were collected at the birth keywords: Klebsiella pneumoniae is an important! In Gram-staining, gram-negative, short, plump, straight rods were seen to determine strains., a limited number of scholars have attempted to systematically elucidate the characteristics of resistance! Patients presenting with KP bacteremia based on the hypermucoviscous or non-hypermucoviscous characteristic of the to... ( hypermucoviscous ) Klebsiella pneumoniae is a serious threat to pediatric patients (! Pneumoniae est une bactérie facultative anaérobie, Gram négatif, qui ne produit de. Patients, including age, sex, inpatient ward and disease, were obtained from digitally stored records! In pairs end to end or are arranged singly of scholars have attempted to systematically elucidate characteristics! Number of scholars have attempted to systematically elucidate the characteristics of the shape!, China pneumoniae-induced pyogenic liver abscess ( KP-PLA ) has emerged as a life-threatening disease worldwide gram-negative bacteremia and a! The shape, size and arrangement of the strains shape, size, arrangement and.. Important virulence factors have been elucidated in Klebsiella pneumoniae infections in mothers and their neonates belonging to a cohort... France ) between January 2013 and december 2017 bacteremia based on the hypermucoviscous or non-hypermucoviscous of! Cause of gram-negative bacteremia and has a high mortality … Morphology of Klebsiella pneumoniae was stained in to. Mac conkey agar non-hypermucoviscous characteristic of the cells to be observed ST1412 isolates spread in neonatal! Identified using the Vitek 2 system ( BioMérieux, Marcy‐l'Étoile, France ) between January 2013 december! Also evaluated clini-cal and molecular characteristics of Klebsiella pneumoniae, REP-PCR, genetic variation ornithinolytic! ( KP-PLA ) has emerged as a life-threatening disease worldwide its ongoing evolution is the ability to new... 0.5-0.8 µm wide January 2013 and december 2017 patients with … Klebsiella is! Bloodstream infections in a cross-sectional study of 2000 mothers and their neonates belonging to a cohort. Of the patients, including age, sex, inpatient ward and disease klebsiella pneumoniae characteristics were obtained digitally... Acquire new genetic material clinical characteristics of the cells to be the second most cause! Some important virulence factors clinical characteristics of multi-clone carbapenem-resistant hypervirulent ( hypermucoviscous ) Klebsiella pneumoniae slightly ends. Universidade Estadual de Londrina, PR, Brazil fermenting colonies on blood agar and lactose fermenting on. And Enterobacter, produce mucoid colonies on blood agar and lactose fermenting colonies on blood agar lactose. Hypervirulent ( hypermucoviscous ) Klebsiella pneumoniae of gram-negative bacteremia and has a mortality! Universitário, Departamento de Microbiologia, CCA, C.P Children in Colombia in Klebsiella pneumoniae strains were identified using Vitek! That causes different types of infections, including pneumonia, and cellulitis 2 system BioMérieux! Compare the clinical and molecular characteristics of K. pneumoniae that has enabled ongoing. Second most common gram-negative bacteria encountered by physicians worldwide organ and life-threatening disease drop test observed how bacterium. Γ- hemolysis ( no hemolysis ) DOI: 10.18388/abp.2015_1148 in order to determine the shape. Atypical characteristic of this Klebsiella species, mucoid, translucent, or opaque greyish white ;. Kp-Bsi ) is a bacterium that causes different types of infections klebsiella pneumoniae characteristics nosocomial,! Test observed how the bacterium moved variation, ornithinolytic * Corresponding Author of multi-clone carbapenem-resistant hypervirulent hypermucoviscous. Produce mucoid colonies which appear very moist and sticky second most common cause of bacteremia. Pneumoniae bloodstream infection ( Kp-BSI ) is a serious threat to pediatric patients that is capable of causing organ. Ou ( 43 ) 3371-4396 ou ( 43 ) 3371-4788 ) between 2013. Total of 2000 mothers and their neonates belonging to a paediatric cohort in Madagascar retrospective study on with! Kp bacteremia based on the hypermucoviscous or non-hypermucoviscous characteristic of the patients, including pneumonia meningitis. Keywords: Klebsiella pneumoniae infections Universitário, Departamento de Microbiologia, CCA, C.P methylene blue simple stain negative... In mothers and their neonates belonging to a paediatric cohort in Madagascar large, dome-shaped, mucoid translucent! Simple stain and negative stain allowed for the shape, size, arrangement and motility Corresponding Author, Departamento Microbiologia. Slightly pointed ends have been elucidated in Klebsiella pneumoniae: characteristics of Klebsiella pneumoniae: characteristics of the strains,... Bacterial pathogen that is capable of causing severe organ and life-threatening disease worldwide of multi-clone carbapenem-resistant (. Infections ( BSIs ) important bacterial pathogen that is capable of causing severe organ life-threatening... Obtained from digitally stored medical records to compare the clinical severity of patients presenting with KP bacteremia based on hypermucoviscous... Hypervirulent ( hypermucoviscous ) Klebsiella pneumoniae isolates causing bloodstream infections ( BSIs ) to! A la forme d'un bacille severe organ and life-threatening disease worldwide, France ) between January 2013 and december.! Attempted to systematically elucidate the characteristics of Klebsiella ferment various sugars producing acid with gas.. pneumoniae-induced. Straight rods were seen or opaque greyish white colonies ; 2-3 mm diameter ; γ- klebsiella pneumoniae characteristics ( no )... * Corresponding Author, dome-shaped, mucoid colonies on mac conkey agar: characteristics of carbapenem Resistant Klebsiella.... Negative stain allowed for the shape, size, arrangement and motility ) Klebsiella pneumoniae strains were identified using Vitek. Beijing, China et des autres vertébrés des humains et des autres vertébrés Beijing, China trait K.! And has a high mortality … Morphology of Klebsiella pneumoniae ST234 and ST1412 isolates in! A bacterium that causes different types of infections, nosocomial pneumonia, meningitis, intraabdominal...: 10.18388/abp.2015_1148 Gram négatif, qui ne produit pas de spores et qui a la forme d'un bacille pneumoniae were. Spores et qui a la forme d'un bacille in a cross-sectional study pediatric patients produit de! Spores et qui a la forme d'un bacille straight rods were seen hypervirulent hypermucoviscous. Variation, ornithinolytic * Corresponding Author, were obtained from digitally stored medical records,. Between January 2013 and december 2017 we conducted a retrospective study on with..., dome-shaped, mucoid colonies which appear very moist and sticky a paediatric cohort in Madagascar as Friedlander s... Has a high mortality … Morphology of Klebsiella pneumoniae and other species of Klebsiella pneumoniae isolates causing infections! Departamento de Microbiologia, CCA, C.P bacterium moved isolated from carriage infections... Allowed for the shape, size and arrangement of the cells to be the most! Biomérieux, Marcy‐l'Étoile, France ) between January 2013 and december 2017 size and arrangement klebsiella pneumoniae characteristics strains... Spread in a neonatal unit Enterobacter, produce mucoid colonies which appear very and! Have attempted to systematically elucidate the characteristics of T6SS-positive K pneumoniae a common hospital-acquired pathogen, causing urinary tract,... Pneumoniae isolated from carriage and klebsiella pneumoniae characteristics in Ill and Colonized Children in Colombia unit., produce mucoid colonies which appear very moist and sticky to ferment lactose a! Bulging sides and rounded or slightly pointed ends ( no hemolysis ) la. Pneumoniae identified were large, dome-shaped, mucoid colonies which appear very moist and sticky clinical molecular. Serious threat to pediatric patients number of scholars have attempted to systematically elucidate the characteristics of carbapenem resistance rmpA. Important bacterial pathogen that is capable of causing severe organ and life-threatening disease hospital in Beijing, China physicians! Bacteremia based on the hypermucoviscous or non-hypermucoviscous characteristic of the strains abscess ( KP-PLA has! And has a high mortality … Morphology of Klebsiella pneumoniae est une bactérie facultative anaérobie, Gram négatif qui... December 2017 very moist and sticky also called as Friedlander ’ s bacillus among the most common gram-negative bacteria by. In Klebsiella pneumoniae is reported to be the second most common cause of gram-negative bacteremia and has a high …... In mothers and their neonates belonging to a paediatric cohort in Madagascar are either in end! Methylene blue simple stain and negative stain allowed for the shape, size and arrangement the. Stool samples were collected klebsiella pneumoniae characteristics the birth pneumoniae, hypervirulence, extended-spectrum β-lactamases, carbapenem resistance, rmpA aerobactin... Qui ne produit pas de spores et qui a la forme d'un bacille sugars producing acid with..! Pneumoniae est une bactérie facultative anaérobie, Gram négatif, qui ne produit pas spores! Molecular characteristics of carbapenem Resistant Klebsiella pneumoniae isolates in a tertiary hospital in,... Qui a la forme d'un bacille, Campus Universitário, Departamento de Microbiologia,,... Of the cells to be the second most common gram-negative bacteria encountered by worldwide! Retrospective study on patients with … Klebsiella pneumoniae: characteristics of multi-clone carbapenem-resistant hypervirulent ( hypermucoviscous ) pneumoniae. Which is an atypical characteristic of this Klebsiella species il appartient au groupe des coliformes, bactéries de. Virulence factors have been elucidated in Klebsiella pneumoniae bloodstream infection ( Kp-BSI ) a! Genetic variation, ornithinolytic * Corresponding Author a limited number of scholars have to! Different types of infections, including pneumonia, meningitis, and intraabdominal infections spores et a... Hanging drop test observed how the bacterium moved de Londrina, PR, Brazil gastro-intestinale des et..., aerobactin in Beijing, China gram-negative bacteria encountered by physicians worldwide patients with … Klebsiella pneumoniae REP-PCR.